HVIDB-PPI detail

P84022 (SMAD3)

Homo sapiens

P27958 (NA)

Hepatitis C virus genotype 1a (isolate H) (HCV)

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Interaction information
PPI score
Human complex
DEGs post infections
Tissue specific expression

Human protein: SMAD3_HUMAN

UniProtID: P84022

Gene name(s): SMAD3;MADH3

Protein name: Mothers against decapentaplegic homolog 3

Gene ID: 4088

HDF/HRF: NA

Virus protein: POLG_HCVH (HCV-H)

UniProtID: P27958

Gene name(s): NA

Protein name: Genome polyprotein

Gene ID: NA

Viral family: Flaviviridae

Interaction	Source database	PubMed ID	Detection method
P84022-P27958	biogrid,HPIDB	23781096	two hybrid

Evidence suggesting a protein interaction:

Interolog evidence:	none/insignificant.
DDI evidence:	none/insignificant.
Machine learning evidence:	yes (score 0.150).

Combined Score: 0.128

Virally targeted human complex

Complex id	Complex name	Complex size	Target ratio	PudMed ID	Source	Detail
1474	SMAD3/4-E2F4/5-p107-DP1 complex	6	0.17 (1/6)	12150994	CORUM	View
1826	SMAD3-HEF1-APC10-CDH1 complex	4	0.25 (1/4)	15144564	CORUM	View
1827	PML-SMAD2/3-SARA complex	4	0.75 (3/4)	15356634	CORUM	View
1828	TGF-beta receptor I-Axin-SMAD3 complex	3	0.33 (1/3)	11438668	CORUM	View
1831	PIAS3-SMAD3-P300 complex	3	0.67 (2/3)	14691252	CORUM	View
2189	Ubiquitin E3 ligase (SMAD3, BTRC, CUL1, SKP1A, RBX1)	5	0.20 (1/5)	11359933	CORUM	View
2692	SMAD3-SMAD4-cJun-cFos complex	4	0.75 (3/4)	9732876	CORUM	View
2705	SMAD3-SMAD4-CTCF protein-DNA complex	3	0.33 (1/3)	12099698	CORUM	View
2706	SMAD3-SMAD4-SP1 complex	3	0.33 (1/3)	11432852	CORUM	View
2707	SMAD3-SMAD4-FOXO3-FOXG1 complex	4	0.25 (1/4)	15084259	CORUM	View
2708	SMAD3-SMAD4-cJUN complex	3	0.67 (2/3)	10220381	CORUM	View
2754	JUND-FOSB-SMAD3-SMAD4 complex	4	0.25 (1/4)	10942775	CORUM	View
2760	SMAD3-SMAD4-FOXO3 complex	3	0.33 (1/3)	15084259	CORUM	View
2761	SMAD3-SMAD4-FOXO1 complex	3	0.33 (1/3)	15084259	CORUM	View
2762	SMAD3-SMAD4-FOXO4 complex	3	0.33 (1/3)	15084259	CORUM	View
2813	BRCA1-SMAD3 complex	2	0.50 (1/2)	15735739	CORUM	View
2829	RSmad complex	10	0.30 (3/10)	16751102	CORUM	View
2830	TIF1gamma-SMAD2-SMAD3 complex	3	0.67 (2/3)	16751102	CORUM	View
2834	SMAD4-SMAD2-SMAD3 complex	3	0.67 (2/3)	16751102	CORUM	View
2968	Axin-SMAD3 complex	2	0.50 (1/2)	11438668	CORUM	View
2975	SMAD3-E2F4/5-p107-DP1 complex	5	0.20 (1/5)	12150994	CORUM	View
3199	SKI-SMAD3 complex	2	0.50 (1/2)	10485843	CORUM	View
3205	SMAD3-SKI-NCOR complex	3	0.33 (1/3)	10485843	CORUM	View
3733	SKI-SMAD3 complex	2	0.50 (1/2)	17283070	CORUM	View
3740	SKI-SMAD3-SMAD4 complex	3	0.33 (1/3)	17283070	CORUM	View
3750	CREBBP-SMAD3 hexameric complex	2	1.00 (2/2)	17283070	CORUM	View
3754	CREBBP-SMAD3-SMAD4 pentameric complex	3	0.67 (2/3)	17283070	CORUM	View
3959	SKI-SMAD3-SMAD4 complex, TGF(beta)-dependent	3	0.33 (1/3)	10575014	CORUM	View
3961	SMAD3-cSKI-SIN3A-HDAC1 complex	4	0.25 (1/4)	10575014	CORUM	View
3972	Ubiquitin E3 ligase (SMURF2, SMAD3) - SnoN complex, TGF(beta)-dependent	3	0.67 (2/3)	11389444	CORUM	View
5735	TGF-beta receptor-SMAD3 complex	3	0.33 (1/3)	8774881	CORUM	View
6649	SMAD3-SMAD4-WWTR1 complex	3	0.33 (1/3)	18568018	CORUM	View
2965	MAN1-SMAD2 complex	4	0.50 (2/4)	NA	hu.MAP	View

Differentially expression condition

Series	Control	Treatment	Regulation	Log2FC	Adj.P.Val	Data type
GSE102481	mock_infected_18h	RVFV_MP12_infected_18h	down	-1.57	1.31e-15	RNA-Seq data
GSE13637	HUVEC_control	PR8-infected_IKK2kd-transfected_HUVEC	down	-1.53	9.20e-05	Microarray data
GSE28166	mock_infected_18h	VN1203_infected_18h	down	-2.32	5.21e-04	Microarray data
GSE28166	mock_infected_24h	VN1203_infected_24h	down	-2.84	1.08e-04	Microarray data
GSE32155	CRL-5800_unifected	CRL-1472_infected	down	-2.53	2.15e-05	Microarray data
GSE32155	CRL-5810_unifected	CRL-5807_infected	up	4.96	7.06e-07	Microarray data
GSE40711	mock_infected_6h	IDN3006_or_cl2PA_infected_6h	up	1.75	8.69e-05	Microarray data
GSE46041	iPS-derived_neurons_uninfected	iPS-derived_neurons_HSV-1-infected	down	-1.53	1.18e-03	Microarray data
GSE49840	Hu_Mock_pCalu3_24h	Hu_VN1203_pCalu3_24h	down	-1.57	1.01e-05	Microarray data
GSE59226	Control	Virus_infection	down	-1.73	2.64e-04	Microarray data
GSE99298	mock_infected_0h	hRSV_NS1_L132A_L133A_infected_96h	up	6.70	7.26e-15	RNA-Seq data
GSE99298	mock_infected_0h	hRSV_NS1_Y125A_infected_96h	up	6.34	2.39e-14	RNA-Seq data
GSE89008	HTBE_H5N1_MOI0_mock_infected_12h	HTBE_H5N1_MOI5_infected_12h	down	-1.73	7.46e-14	RNA-Seq data
GSE89008	HTBE_H5N1_MOI0_mock_infected_18h	HTBE_H5N1_MOI5_infected_18h	down	-2.15	8.68e-17	RNA-Seq data
GSE97672-GPL16791	MDM-315_H1N1_MOI0_18h	MDM-315_H1N1_MOI2_18h	up	2.83	9.50e-03	RNA-Seq data
GSE97672-GPL16791	MDM-520_H5N1_MOI0_12h	MDM-520_H5N1_MOI2_12h	up	1.79	1.57e-02	RNA-Seq data
GSE97672-GPL16791	MDM-520_H5N1_MOI0_18h	MDM-520_H5N1_MOI2_18h	up	1.63	2.15e-02	RNA-Seq data
GSE97672-GPL18573	MDM-513_H5N1_MOI0_12h	MDM-513_H5N1_MOI2_12h	up	3.48	9.52e-05	RNA-Seq data

Human tissue specific expression (TPM)

Expression value is measured by Transcripts Per Million (TPM)

Low tissue specificity

Ensembl ID:	ENSG00000166949
Gene description:	SMAD family member 3
Chromosome:	15
Position	67063763-67195195
Protein class:	Cancer-related genes, Disease related genes, Predicted intracellular proteins, Transcription factors
Evidence:	Evidence at protein level
Subcellular location:	Nucleoplasm, Cytosol