JobID: QZ0Z61494947037 - WDRR: WD40 Repeat Recognition

Input parameters:

  Query = readseq-11476_tmp_1 (Len=969)
  Tmplt = WD40-str (Len=40)
  Method = bdp  Gap-open = 50  Gap-extend = 3
  Database = /home/pub/blastdb/nr90  E-value = 0.001  Iteration = 3

Query sequence (readseq-11476_tmp_1, Len=969):

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Query        1 MSQTNWEADKMLDVYIHDYLVKRDLKATAQAFQAEGKVSSDPVAIDAPGGFLFEWWSVFWDIFIARTNEKHSEVAASYIE 80  
WD40-rpts                                                                                      

Query       81 TQMIKAREQQLQQSQHPQVSQQQQQQQQQQIQMQQLLLQRAQQQQQQQQQQHHHHQQQQQQQQQQQQQQQQQQQQHQNQP 160 
WD40-rpts                                                                                      

Query      161 PSQQQQQQSTPQHQQQPTPQQQPQRRDGSHLANGSANGLVGNNSEPVMRQNPGSGSSLASKAYEERVKMPTQRESLDEAA 240 
WD40-rpts                                                                                      

Query      241 MKRFGDNVGQLLDPSHASILKSAAASGQPAGQVLHSTSGGMSPQVQTRNQQLPGSAVDIKSEINPVLTPRTAVPEGSLIG 320 
WD40-rpts                                                                                      

Query      321 IPGRFAVLSVSFQLVKRVKCSISTSAHKNLLRINDWFPVSGSNQGSNNLTLKGWPLTGFDQLRSGLLQQQKPFMQSQSFH 400 
WD40-rpts                    (__________________WD 1_________________)                         

Query      401 QLNMLTPQHQQQLMLAQQNLNSQSVSEENRRLKMLLNNRSMTLGKDGLGSSVGDVLPNVGSSLQPGGSLLPRGDTDMLLK 480 
WD40-rpts                                            (_________________________WD 2____________

Query      481 LKMALLQQQQQNQQQGGGNPPQPQPQPQPLNQLALTNPQPQSSNHSIHQQEKLGGGGSITMDGSISNSFRGNEQVLKNQS 560 
WD40-rpts      _____________)  (_________________WD 3________________)       (_________________

Query      561 GRKRKQPVSSSGPANSSGTANTAGPSPSSAPSTPSTHTPGDVISMPNLPHSGGSSKSMMMFGTEGTGTLTSPSNQLADMD 640 
WD40-rpts      WD 4________________)  (_________________WD 5________________)        (_________

Query      641 RFVEDGSLDDNVESFLSQEDGDQRDAVTRCMDVSKGFTFTEVNSVRASTTKVTCCHFSSDGKMLASAGHDKKAVLWYTDT 720 
WD40-rpts      _________WD 6__________________)      (_________________WD 7________________)   

Query      721 MKPKTTLEEHTAMITDIRFSPSQLRLATSSFDKTVRVWDADNKGYSLRTFMGHSSMVTSLDFHPIKDDLICSCDNDNEIR 800 
WD40-rpts      (_________________WD 8________________)    (_________________WD 9_______________

Query      801 YWSINNGSCTRVYKGGSTQIRFQPRVGKYLAASSANLVNVLDVETQAIRHSLQGHANPINSVCWDPSGDFLASVSEDMVK 880 
WD40-rpts      __)   (_______________WD10_______________)   (________________WD11______________

Query      881 VWTLGTGSEGECVHELSCNGNKFQSCVFHPAYPSLLVIGCYQSLELWNMSENKTMTLPAHEGLITSLAVSTATGLVASAS 960 
WD40-rpts      __)      (_________________WD12________________)(__________________WD13_________

Query      961 HDKLVKLWK 969 
WD40-rpts      ________)

13 WD40-repeats found in QUERY=readseq-11476_tmp_1 (Len=969):

(Red - Z-score>4.0)

  Rpts  Segment   Boundary  Z-score   Score  P-value  Identities  SS-score
  WD 1:  Seg  5   335-375      6.35  376.94  0.00052       12.5%     13.48
  WD 2:  Seg  6   439-494      4.78  311.96  0.00388       27.5%     18.50
  WD 3:  Seg  8   497-535      7.04  405.48  0.00021        7.5%     21.00
  WD 4:  Seg 10   543-581      7.19  411.81  0.00018       10.0%     41.16
  WD 5:  Seg 13   584-622      3.90  275.70  0.01193       12.5%     10.34
  WD 6:  Seg 16   631-672      5.70  349.92  0.00120       15.0%     35.43
  WD 7:  Seg 19   679-717      8.66  472.21  2.7e-05       30.0%     55.55
  WD 8:  Seg 23   721-759     10.02  528.49  4.7e-06       40.0%     56.70
  WD 9:  Seg 27   764-803      8.46  464.21  3.4e-05       40.0%     50.43
  WD10:  Seg 31   807-842      6.87  398.33  0.00027       25.0%     51.11
  WD11:  Seg 35   846-883      8.34  459.12  4.0e-05       37.5%     50.82
  WD12:  Seg 39   890-928      8.25  455.61  4.5e-05       25.0%     57.15
  WD13:  Seg 43   929-969      8.23  454.69  4.6e-05       30.0%     51.10

Multiple sequence alignment of 13 WD40-repeats:

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

   WD 1  335-375    VKR-VKCSISTSAHKNLLRI------NDWF-PVSGSNQGSN----NLTLK-----------GWP
   WD 2  439-494    -RS-M-TLGKDGLGSSVGDVLPNVGSSLQP-GGSLLPRGDT----DMLLKLKMALLQQQQQNQQ
   WD 3  497-535    -GG-N-PPQPQPQPQPLNQL------ALTN-PQPQSSNHSI----HQQEK-----------LGG
   WD 4  543-581    -GS-I-SNSFRGNEQVLKNQ------SGRK-RKQPVSSSGP----ANSSG-----------TAN
   WD 5  584-622    -GP-S-PSSAPSTPSTHTPG------DVIS-MPNLPHSGGS----SKSMM-----------MFG
   WD 6  631-672    SPS----NQLADMDRFVEDG------SLDD-NVESFLSQEDGDQRDAVTR-----------CMD
   WD 7  679-717    -FT-E-VNSVRASTTKVTCC------HFSS-DGKMLASAGH----DKKAV-----------LWY
   WD 8  721-759    -MK-P-KTTLEEHTAMITDI------RFSP-SQLRLATSSF----DKTVR-----------VWD
   WD 9  764-803    -GY-S-LRTFMGHSSMVTSL------DFHPIKDDLICSCDN----DNEIR-----------YWS
   WD10  807-842    -GS-C-TRVYKGG---STQI------RFQP-RVGKYLAASS----ANLVN-----------VLD
   WD11  846-883    -QA-I-RHSLQGHANPINSV------CWDP-SGDFLASVSE----D-MVK-----------VWT
   WD12  890-928    -GE-C-VHELSCNGNKFQSC------VFHP-AYPSLLVIGC----YQSLE-----------LWN
   WD13  929-969    MSENK-TMTLPAHEGLITSL------AVST-ATGLVASASH----DKLVK-----------LWK

   WD40 Template    TGE-C-LRTLSGHTSGVTSV------SFSP-DGKRLVSGSE----DGTIK-----------VWD