JobID: IBQS11620966760 - WDRR: WD40 Repeat Recognition

Input parameters:

  Query = 2AQ5_A_PDBID_CHAIN_SEQUENCE (Len=423)
  Tmplt = WD40-str (Len=40)
  Method = bdp  Gap-open = 50  Gap-extend = 3
  Database = /home/pub/blastdb/nr90  E-value = 0.001  Iteration = 3

Query sequence (2AQ5_A_PDBID_CHAIN_SEQUENCE, Len=423):

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Query        1 MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFMALICEASGGGAFLVLPLGKTGRVDKNVPLVCGH 80  
WD40-rpts                        (____________________WD 1___________________)       (_________

Query       81 TAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLVLPLREPVITLEGHTKRVGIVAWHPTAQNVLLSAGCDNVILVWD 160 
WD40-rpts      ________WD 2_________________)          (_________________WD 3_________________)

Query      161 VGTGAAVLTLGPDVHPDTIYSVDWSRDGALICTSCRDKRVRVIEPRKGTVVAEKDRPHEGTRPVHAVFVSEGKILTTGFS 240 
WD40-rpts         (__________________WD 4_________________)   (___________________WD 5_________

Query      241 RMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMG 320 
WD40-rpts      __________)(____________________WD 6___________________)    (__________________W

Query      321 YMPKRGLEVNKCEIARFYKLHERKCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPK 400 
WD40-rpts      D 7__________________)   (_________________WD 8________________)                

Query      401 SRANDSELECTWAITFORDELAY 423 
WD40-rpts

8 WD40-repeats found in QUERY=2AQ5_A_PDBID_CHAIN_SEQUENCE (Len=423):

(Red - Z-score>4.0)

  Rpts  Segment   Boundary  Z-score   Score  P-value  Identities  SS-score
  WD 1:  Seg  3    19-63       3.77  270.40  0.01405       17.5%     48.06
  WD 2:  Seg  7    71-110      8.18  452.46  5.0e-05       32.5%     49.51
  WD 3:  Seg 11   121-160      7.66  431.28  9.6e-05       40.0%     54.10
  WD 4:  Seg 15   164-204      7.92  441.86  6.9e-05       27.5%     57.07
  WD 5:  Seg 19   208-251      5.55  343.95  0.00144       25.0%     46.19
  WD 6:  Seg 23   252-296      5.87  357.09  0.00096       30.0%     45.11
  WD 7:  Seg 27   301-342      3.53  260.53  0.01904       20.0%     43.21
  WD 8:  Seg 31   346-384      4.05  281.80  0.00988        7.5%     17.67

Multiple sequence alignment of 8 WD40-repeats:

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

   WD 1   19-63     AKADQCYEDVRVS----QTTW--DSGFCA--VNP-KFMA-LICEA---SG-GGAFLVLP
   WD 2   71-110    -DKNV----PLVC----GHTA--PVLDIA--WCPHNDNV-I-ASG---SE-DCTVMVWE
   WD 3  121-160    -REPV----ITLE----GHTK--RVGIVA--WHP-TAQNVL-LSA---GC-DNVILVWD
   WD 4  164-204    -GAAV----LTLG--PDVHPD--TIYSVD--WSR-DGAL-I-CTS---CR-DKRVRVIE
   WD 5  208-251    -GTVV----AEKD----RPHE--GTRPVHAVFVS-EGKI-L-TTGFSRMS-ERQVALWD
   WD 6  252-296    TKHLE----EPLSLQELDTSS--GVLLPF--FDP-DTNI-VYLCG---KG-DSSIRYFE
   WD 7  301-342    -APFL----HYLS----MFSSKESQRGMG--YMP-KRGL-E-VNK---CEIARFYKLHE
   WD 8  346-384    -EPIA----MTVP----RKSD--LFQEDL--YPP-TAGP-D-PAL---TA-EEWLGGRD

   WD40 Template    TGECL----RTLS----GHTS--GVTSVS--FSP-DGKR-L-VSG---SE-DGTIKVWD