JobID: 901OV1620966760 - WDRR: WD40 Repeat Recognition

Input parameters:

  Query = 2AQ5_A_PDBID_CHAIN_SEQUENCE (Len=438)
  Tmplt = WD40-str (Len=40)
  Method = bdp  Gap-open = 50  Gap-extend = 3
  Database = /home/pub/blastdb/nr90  E-value = 0.001  Iteration = 3

Query sequence (2AQ5_A_PDBID_CHAIN_SEQUENCE, Len=438):

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Query        1 MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFMALICEASGGGAFLVLPLGKTGRVDKNVPLVCGH 80  
WD40-rpts                        (____________________WD 1___________________)       (_________

Query       81 TAPVLDIAWCPHNDNVIASGSEDCTVMVWEIPDGGLVLPLREPVITLEGHTKRVGIVAWHPTAQNVLLSAGCDNVILVWD 160 
WD40-rpts      ________WD 2_________________)          (_________________WD 3_________________)

Query      161 VGTGAAVLTLGPDVHPDTIYSVDWSRDGALICTSCRDKRVRVIEPRKGTVVAEKDRPHEGTRPVHAVFVSEGKILTTGFS 240 
WD40-rpts         (__________________WD 4_________________)   (___________________WD 5_________

Query      241 RMSERQVALWDTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSEAPFLHYLSMFSSKESQRGMG 320 
WD40-rpts      __________)(____________________WD 6___________________)(____________________WD 

Query      321 YMPKRGLEVNKCEIARFYKLHERKCEPIAMTVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPK 400 
WD40-rpts      7____________________)   (_________________WD 8________________)                

Query      401 SRANDSELECTFROMSELECTSLEEPAUNIONSELECT 438 
WD40-rpts

8 WD40-repeats found in QUERY=2AQ5_A_PDBID_CHAIN_SEQUENCE (Len=438):

(Red - Z-score>4.0)

  Rpts  Segment   Boundary  Z-score   Score  P-value  Identities  SS-score
  WD 1:  Seg  3    19-63       3.70  267.33  0.01544       17.5%     47.74
  WD 2:  Seg  7    71-110      8.12  450.21  5.3e-05       32.5%     49.51
  WD 3:  Seg 11   121-160      7.64  430.39  9.8e-05       40.0%     53.61
  WD 4:  Seg 15   164-204      7.91  441.53  7.0e-05       27.5%     57.38
  WD 5:  Seg 19   208-251      5.52  342.72  0.00150       25.0%     47.81
  WD 6:  Seg 23   252-296      5.88  357.42  0.00095       30.0%     45.13
  WD 7:  Seg 27   297-342      3.22  247.66  0.02825       10.0%     40.01
  WD 8:  Seg 31   346-384      4.00  279.92  0.01047        7.5%     17.76

Multiple sequence alignment of 8 WD40-repeats:

(BLUE - beta-strands, RED - alpha-helices, predicted by PSIPRED)

   WD 1   19-63     AKA-----DQCYEDVRVS----QTTWDSGFCA--VNP-KFMA-LICEA---SG-GGAFLVLP
   WD 2   71-110    -DK-----NV----PLVC----GHTAPVLDIA--WCPHNDNV-I-ASG---SE-DCTVMVWE
   WD 3  121-160    -RE-----PV----ITLE----GHTKRVGIVA--WHP-TAQNVL-LSA---GC-DNVILVWD
   WD 4  164-204    -GA-----AV----LTLG--PDVHPDTIYSVD--WSR-DGAL-I-CTS---CR-DKRVRVIE
   WD 5  208-251    -GT-----VV----AEKD----RPHEGTRPVHAVFVS-EGKI-L-TTGFSRMS-ERQVALWD
   WD 6  252-296    TKH-----LE----EPLSLQELDTSSGVLLPF--FDP-DTNI-VYLCG---KG-DSSIRYFE
   WD 7  297-342    ITSEAPFLHY----LSMF----SSKESQRGMG--YMP-KRGL-E-VNK---CEIARFYKLHE
   WD 8  346-384    -EP-----IA----MTVP----RKSDLFQEDL--YPP-TAGP-D-PAL---TA-EEWLGGRD

   WD40 Template    TGE-----CL----RTLS----GHTSGVTSVS--FSP-DGKR-L-VSG---SE-DGTIKVWD